PAb Anti Mouse IgG-Tb cryptate
- High affinity
IgGs from Rabbit anti-Mouse immunoserum were immunopurified and labeled with Tb. PAb Anti Mouse IgG-Tb cryptate may be used to detect the binding of unlabeled mouse specific antibody on a target molecule or on Ig fusion proteins.
HTRF can be used in both biochemical and cellular formats to study a wide variety of interactions: protein/protein, protein/peptide, protein/DNA, protein/RNA, protein/carbohydrate, protein/small molecule, receptor/ligand.
HTRF can detect a broad range of affinity constants ranging from picomolar to low millimolar.
In an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the signal is proportional to the binding of the 2 partners.
In the example shown here: PAb Anti Mouse IgG-Tb cryptate binds to the Mouse Fc fused tagged partner A while partner B* binds to a specific Ab labeled with an HTRF acceptor.
*partner B can also be biotinylated, tagged, Fc fused. In these cases, use the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin) labeled with acceptor for the detection.
The example on the right describes the protocol using a 20 µL final assay volume for detecting an interaction between a Mouse Fc fused-tagged partner A and a non-tagged partner B*.
Dispense the 2 partners (10 µL), incubate, add PAb Anti Mouse IgG-Tb cryptate (5 µL) and anti-partner B labeled with acceptor (5 µL), incubate and read.
*partner B can also be biotinylated, tagged, Fc fused or directly labeled. In these cases, use the corresponding HTRF reagent (anti-Tag, anti species, protA, Streptavidin) labeled with acceptor for the detection.
61PAMTAF-61PAMTAA-61PAMTAB - Product Insert
Easy pharmacological characterization of PPI modulators. - Technical Notes
Deciphering low- and high affinity interactions - Application Notes
Monitoring nuclear receptor binding with HTRF assays - Application Notes
Challenge large complexes with HTRF assays - Application Notes