XL665-labeled Streptavidin for capturing biotinylated proteins, peptides, antibodies, small molecules, nucleic acids....
- High affinity
Streptavidin has been labeled with XL665. Biotin binds to Streptavidin with high affinity (Ka=1015M-1). The binding is rapid and stable, making it an ideal choice for use in a variety of assays such as enzyme assays, protein-protein binding assays and molecular biology assays.
The example on the right describes the protocol using a 20 µL final assay volume for detecting an interaction between a biotinylated-tagged partner A and a non-tagged partner B*.
Dispense the 2 partners (10 µL), incubate, add Streptavidin-XL (5 µL) and anti-partner B labeled with donor (5 µL), incubate and read.
*partner B can also be tagged, Fc fused or directly labeled. In these cases use for the detection, the corresponding HTRF reagent (anti-Tag, anti species, protA, Streptavidin), labeled with donor.
In collaboration with Lead Discovery Center GmbH - Scientific Presentations
Benefit from unlimited flexibility for your assay development - Flyers
In collaboration with Boehringer Ingelheim - Posters
In collaboration with Universidad de Navarra - Posters
Every interaction is within range - Technical Notes
610SAXLF-610SAXLA-610SAXLB - Product Insert
Easy pharmacological characterization of PPI modulators. - Technical Notes
Deciphering low- and high affinity interactions - Application Notes
Monitoring nuclear receptor binding with HTRF assays - Application Notes
Challenge large complexes with HTRF assays - Application Notes