Biotin-Heparan sulfate-Eu cryptate reagent
HTRF heparan sulfate substrate labeled with both biotin and Eu3+ Cryptate used with HTRF SA-XL665, allows to monitor heparanase activity with the robustness of HTRF technology.
Heparanase is an enzyme that cleaves heparan sulfate, and thereby releases growth factors that are implicated in tumor cell proliferation, metastasis and angiogenesis.
Heparanase assay was performed using a range of enzyme concentrations with a final assay volume of 20 µL. Then an inhition assay was performed using heparanase at 90ng/mL incubated for 30 minutes at RT in presence of various concentrations of Suramin.
Fig A shows the Heparanase assay standard curve, assay CVs were lower than 3%. Fig.B shows the inhibitory effect of Suramin, a known inhibitor of heparanases. The IC50 was calculated at 4 µM, which is in agreement with values calculated using radiometric methods (1-10µM).
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