The Mouse IL1 beta kit is designed for the quantification of mouse IL1 beta release in cell supernatant.
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  • No-wash
  • Low sample consumption
  • Under 2h bench time
The Mouse IL1 beta kit is designed for the quantification of mouse IL1 beta release in cell supernatant.
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In stock

Overview

IL1 beta, also known as leukocytic pyrogen or mononuclear cell factor, is a pro-inflammatory cytokine of the IL1 family. IL1 beta is considered to be a major endogenous pyrogen and induces prostaglandin synthesis, T cell activation, B cell activation, and subsequent antibody production. It is also a known promoter of T cell differentiation into Th17.

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL1 beta analysis.

Technical specifications of mouse IL1 beta kit

Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent) 14 pg/mL &46 pg/mL
Range 46 – 4,200 pg/mL
Time to result ON at RT
Calibration NIBSC (93/668) value (IU/mL) = 0,1 x HTRF mIL1ß value (pg/mL)
Species Mouse only

Intra and inter assay

Intra-assay (n=24)

Sample Mean [IL1ß] (pg/mL) CV
1 100 6%
2 800 2%
3 3200 2%
Mean CV 3%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample[IL1ß] (pg/mL)Mean (delta R)CV
11702722%
261210234%
3221130091%


Mean CV2%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample

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