GLP1R fluorescent probe

This GLP1 receptor red agonist is an Exendin4 derivative labeled with a red emitting HTRF fluorescent probe.
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  • Non-radioactive Non-radioactive
  • No-wash No-wash
  • Cell-based Cell-based
  • Equilibrium & Kinetic binding compatible Equilibrium & Kinetic binding compatible
This GLP1 receptor red agonist is an Exendin4 derivative labeled with a red emitting HTRF fluorescent probe.
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Overview

Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function.

This GLP1 receptor red agonist is an Exendin4 derivative labeled with a red emitting HTRF fluorescent probe.

This ligand is best suited for use in the Glucagon GLP1 receptor binding assay.

Benefits

  • FLUORESCENTLY LABELED LIGAND
  • HIGHLY STABLE
  • VALIDATED IN BINDING

Assay principle

Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Diagram of a receptor binding assay using the Tag-lite protocol

Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Diagram of a saturation binding assay using Tag-lite
Representative data obtained when running a saturation binding assay

Watch this video explaining how to run a saturation binding assay using Tag-lite.

Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Diagram of a competitive binding assay using Tag-lite
Representative data obtained when running a competitive binding assay

Watch this video explaining how to run a competitive binding assay using Tag-lite.

Evaluation of a Tag-lite binding assay for a class B receptor

In collaboration with Boehringer Ingelheim - Scientific Presentations

Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery

In collaboration with Bayer - Scientific Presentations

Tag-lite binding assays

Benefit from them to move away from radioactivity - Flyers

Understanding GPCRs is the key to improved DD

In collaboration with GEN - Guides

Tag-lite, the easiest solution for studying GPCR-ligands interactions

The gold standard technology for receptor binding studies - Videos

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

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