Glucagon quantification in cell supernatant
The Insulin Ultra-Sensitive kit is designed for the quantification of insulin in low-concentration samples of cell supernatant. An excellent tool in diabetes research.
One hurdle faced by researchers seeking to quantify insulin is the high number of islets often required per assay. Designed for enhanced sensitivity, this kit is intended for the quantification of insulin in low-concentration samples. The kit is ideal for the detection of basal insulin levels and insulin released from mouse pancreatic islets.
Pancreatic islets were isolated from C57BL/6N male mice. The islets were dispensed into tubes with KRB buffer containing either 2.8 mM or 16.7 mM glucose (5 islets/tube, 6 tubes/condition). After 10 minutes or 30 minutes of incubation, the supernatants* were collected and the insulin concentrations measured using the HTRF Ultra-sensitive insulin kit reagents.
*Samples were kindly provided by S. Dalle and M. Ravier’s research team: Physiopathology of pancreatic beta-cells, Institute for Functional Genomics (IGF), Montpellier, France.
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