cAMP Gs dynamic kit
Optimal performance when applied to Gs activation or inhibition
The phospho-CREB (S133) kit is optimal for the quantitative detection of the cyclic AMP-responsive element-binding protein when phosphorylated at S133, thus enabling the phosphorylation status of CREB to be checked with respect to its steady-state level in cells. CREB functions as a transcription factor, signaling downstream from MAPK/ERK and activated by several pathways, such as MAPK/ERK, PI3K/AKT and stress signaling. CREB plays a critical role in promoting neuronal plasticity, cognitive abilities, cell proliferation, survival, and glucose homeostasis.
Mouse pancreatic ß-cells Min6 are seeded in culture-treated 96-well plates at 70Kc/well and cultured for 6 days in complete culture medium at 37°C, 5% CO2. After a cell washing step with KRB (Krebs Ringer Buffer) and a quiescence step for 2h in KRB, cells were stimulated with 50 µL KRB and increasing concentrations of Glucose for 5 min at 37°C. After a 30 minutes lysis incubation time, total and phospho-CREB were measured using the two-plate assay protocol.
Murine NIH 3T3 cells (50,000 cells/well) were stimulated for 30 minutes at 37°C with various concentrations of forskolin. After a 30 minutes lysis incubation time, phosphorylated CREB was measured using the two-plate assay protocol.
A431 cells (100,000 cells/well) were incubated for 30 min at 37°C with various concentrations of antagonist. Agonist (EGF) was then added and incubated for 10 minutes. After 30 minutes lysis incubation time, phosphorylated CREB was measured using the two-plate assay protocol.
Analyse of PI3K/AKT/mTor translational control pathway - Application Notes
Changes in CREB and ERK phosphorylation levels after beta-amyloid treatment - Application Notes
In collaboration with Bayer - Scientific Presentations
Cell Signaling: Biomarkers, Phospho- & total-protein Assays - Flyers
Physiologically relevant results fo fast flowing research - Flyers
HTRF products and supporting scientific content to investifgate GPCRs - Flyers
Do all cell-based kinase assays perform similarly? - Posters
PI3K/AKT/mTor translational control pathway - Posters
Analysis of a large panel of diverse biological samples and cellular models - Posters
Multidimentional GPCR investigations - Posters
Study a pathway of interest in PBMC and T cells - Application Notes
One technology across all samples - Application Notes
Tumor xenograft analysis: HTRF versus Western blot - Application Notes
Valuable guidelines for efficiently analyzing and interpreting results - Application Notes
Increased flexibility of phospho-assays - Application Notes
16 GPCR challenges, 1 solution - Posters
Multi-tissue cellular modeling and anlysis of insulin signaling - Posters
Insider Tips for successful sample treatment - Technical Notes
HTRF and WB compatible guidelines - Technical Notes
Mastering the art of cell signaling assays optimization - Guides
Detailed protocol and direct comparison with WB - Posters
A single technology for 2D cells, 3D cells, and xenograft models - Posters
Protocol for tumor xenograft analysis with HTRF - Technical Notes
Unmatched ease of use, sensitivity and specificity assays - Videos
Get the brochure about technology comparison. - Brochures
A solution for phospho-protein analysis in metabolic disorders - Posters
A fun video introducing you to phosphorylation assays with HTRF - Videos