This HTRF kit detects changes of cAMP accumulation in response to Gs coupled GPCR activation or inhibition

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  • Ease-of-use Ease-of-use
  • High sensitivity High sensitivity
  • Accurate pharmacology Accurate pharmacology

This HTRF kit detects changes of cAMP accumulation in response to Gs coupled GPCR activation or inhibition

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In stock

Overview

Based on HTRF technology, the kit contains all the reagents needed to quantify cAMP, ensuring that you benefit from a complete tool for your needs.

Cisbio's cAMP kits are best suited for measuring cAMP  modulations in response to Gs-activation, leveraging an optimized combination of robustness and sensitivity

Benefits

  • SCREENING OF GS COUPLED GPCR
  • LEAD OPTIMIZATION OF GS COMPOUNDS
  • STUDY OF GS GPCR RESPONSES

Assay principle

Cisbio cAMP kits are based on a competitive format involving a specific antibody labeled with cryptate (donor) and cAMP coupled to d2 (acceptor). This enables the direct characterization of all types of compounds acting on Gs-coupled receptors in either adherent or suspension cells.
Diagram of cAMP Gs Hirange assay principle

Assay protocol

The protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents
Diagram of cAMP Gs Hirange assay protocol

cAMP Gs hirange product specifications

If your experimental design uses membrane preparations, lipoparticles or purified receptors, or if your samples have large quantities of cAMP, the Gs HiRange kit will fit your needs! 

The kit shows no ATP cross-reactivity up to 10mM, allowing the user to operate with large ranges of ATP and enabling membrane work.

Designed for optimal signal performance, cAMP - Gs HiRange offers a maximized signal-to-background score.

cAMP Gs hirange standard curve characteristics
                                                        
S/BIC20 nMIC50 nMIC80 nM
cAMP - Gs dynamic>140.914.0718.1
cAMP - Gs HiRange>284.2722.8121

ATP cross-reactivity

No ATP cross-reactivity up to 10 mM
No ATP cross-reactivity up to 1 mM
No ATP cross-reactivity up to 1 mM
HTRF assays with iPSC derivated cell types

In collaboration with Cellular Dynamics International and BMG labtech - Scientific Presentations

Structure, function and pharmacology of G protein-coupled receptors: State-of-the-art and future challenges

In collaboration with the Faculty of health and medical sciences Denmark - Scientific Presentations

HTRF assays of IP1, cAMP and pERK pathways employed to study biased signaling of the calcium-sensing receptor

In collaboration with the Faculty of health and medical sciences Denmark - Scientific Presentations

HTRF a versatil approach for 7TM drug discovery

In collaboration with MRC - Scientific Presentations

cAMP Fresh and Frozen Cell Usage

In collaboration with Bristol Myers Squibb - Scientific Presentations

IP-One & cAMP HTRF Functional Cell-based Screening

In collaboration with Schering AG - Scientific Presentations

GPCR research from A to Z

HTRF products and supporting scientific content to investifgate GPCRs - Flyers

Application of Bioassay Performance Monitoring Process with Statistical Tools

In collaboration with Amylin Pharmaceuticals Inc., Biocon Limited - Posters

Characterisation of Inositol Phosphate & cAMP Signalling from GPCRs using HTRF

In collaboration with College of Science, King Saud University, University of Western Australia - Posters

HTRF® technology on the SpectraMax® i3 Multi-Mode Platform

In collaboration with Mollecular Devices - Posters

Kinetics of the ß2 Adrenergic receptor binding and signaling

A look at the kinetics of cAMP production in cells - Application Notes

Ultimate guides to master Gs and Gi GPCR assays

Optimizing cAMP assays has never been easier - Guides

Enhanced performance of the new HTRF® cAMP kit on Gai/o coupled GPCR

Kit specifically designed to investigate Gai/o signaling - Posters

Understanding GPCRs is the key to improved DD

In collaboration with GEN - Guides

How to run a cAMP HTRF assay

What to expect at the bench - Videos

Product Insert cAMP Gs HiRg / 62AM6PEB-62AM6PEC-62AM6PEJ

62AM6PEB-62AM6PEC-62AM6PEJ - Product Insert

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

GPCR signaling in cardiovascular research addressed with HTRF 2nd messenger assays

See how peer researchers monitor GPCR signaling with HTRF - Application Notes

Molecular basis of neuroinflammation and neurodegeneration diseases

The essential guide for extending your knowledge on the molecular mechanisms of neurodegenerative diseases - Guides

Safety Data Sheet cAMP Gs HiRg / 62AM6PEB

62AM6PEB - Safety Data Sheet

Safety Data Sheet cAMP Gs HiRg / 62AM6PEC

62AM6PEC - Safety Data Sheet

Safety Data Sheet cAMP Gs HiRg / 62AM6PEJ

62AM6PEJ - Safety Data Sheet

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