Serotonin 5HT1AR expressing cells labeled with Terbium

Tag-lite cells transiently expressing the Serotonin 5HT1A receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
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  • Fully functional cell line Fully functional cell line
  • Transient expression Transient expression
  • Ready for binding Ready for binding
Tag-lite cells transiently expressing the Serotonin 5HT1A receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.
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Overview

The 5-HT1A receptor is a GPCR widely distributed in regions that receive serotonergic input, including the frontal cortex, septum, amygdala, hippocampus, and hypothalamus. The serotonin 1A receptor (5-HT1A) plays a role in the genesis of several major depressive disorders.

Cells expressing the 5HT1A receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.

Benefits

  • PRE-LABELED WITH TERBIUM
  • FROZEN CELLS, READY TO BE USED
  • CELLS/LIGAND VALIDATED FOR BINDING

Assay principle

Running your 5HT1A receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

Diagram of 5HT1A receptor binding assay using the Tag-lite protocol

Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Diagram of a saturation binding assay using Tag-lite
Representative data obtained when running a saturation binding assay

Watch this video explaining how to run a saturation binding assay using Tag-lite.

Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Diagram of a competitive binding assay using Tag-lite
Representative data obtained when running a competitive binding assay

Watch this video explaining how to run a competitive binding assay using Tag-lite.

Kd and Ki validation

Examples of data obtained using Serotonin 5HT1A labeled cells and their matching fluorescent ligand (L0029RED). WAY-100135 was used as reference ligand. Results may vary from one HTRF® compatible reader to another.

Representative saturation binding graph
Representative competitive binding graph
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