Halo tag A2A AdenosineR cloned plasmid

The Tag-lite A2A Adenosine plasmid is used to transiently or stably transfect cells for the purpose of developing a A2A Adenosine receptor binding assay.
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The Tag-lite A2A Adenosine plasmid is used to transiently or stably transfect cells for the purpose of developing a A2A Adenosine receptor binding assay.
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Overview

Over the past few years, SNAP/HALO-Tag technology combined with TR-FRET has paved the way for the development of many non-radioactive, no-wash, binding assays.

The method is based on transfecting cells using plasmids encoding a SNAP- or HALO-Tag and subsequently labeling them with Terbium. Cisbio offers a large collection of such plasmids. All GPCR genes are cloned in an expression vector directly downstream from a CMV promoter, and are provided ready for protein expression and labeling.

All information on this page pertains to the Tag-lite plasmid cloned with the A2A Adenosine receptor.

Benefits

  • FULL LENGTH RECEPTOR
  • HIGH EXPRESSION SYSTEM
  • VALIDATED IN EXPRESSION
Evaluation of a Tag-lite binding assay for a class B receptor

In collaboration with Boehringer Ingelheim - Scientific Presentations

Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery

In collaboration with Bayer - Scientific Presentations

Tag-lite binding assays

The easy way to approach biology without radioactivity - Flyers

Understanding GPCRs is the key to improved DD

In collaboration with GEN - Guides

Tag-lite, the easiest solution for studying GPCR-ligands interactions

The gold standard technology for receptor binding studies - Videos

Transient Transfection Procedure

Cell transfection protocol - Product Insert

Labeling procedure Tag-lite

Cell surface receptor labeling - Product Insert

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

Kinetic adenosine receptor family binding assays

Case study about kinetic binding characterization provided by the combination of Cisbio and Excellerate bioscience expertise. - Application Notes

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Easy method for kinetic binding determination

How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes

Safety Data Sheet pHalo-A2A-R / PHTA2A

PHTA2A - Safety Data Sheet

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