Estradiol quantification from cells, serum and enzymatic assays
The HTRF cynomolgus TNF alpha kit is intended for the quantification of cynomolgus TNF alpha release in cell supernatant.
TNF alpha, mainly produced by activated macrophages, is one of the main cytokines in acute phase reaction. Depending on the cellular target and the biological context, TNF alpha can activate pro-apoptosis or pro-survival pathways, thus regulating tissue development through apoptosis control. Outside of oncology and inflammation, TNF alpha contributes to the development of type II diabetes by increasing insulin resistance and modulating fatty acid metabolism.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Cynomolgus TNFα AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your TNF alpha analysis.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers & protocol.|
|LOD & LOQ (in Diluent)||12.4 pg/mL & 27 pg/mL|
|Range||27 – 3,000 pg/mL|
|Time to result||ON at RT|
|Sample||Mean [TNFa] (pg/mL)||CV|
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
|Sample||[TNFa] (pg/mL)||Mean (delta R)||CV|
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
In collaboration with CNRS - Scientific Presentations
In collaboration with Molecular Devices - Application Notes
In collaboration with Blood assay solution - Application Notes
Perform and optimize cytokine assays on PBMC - Technical Notes
A fun video introducing you to cytokines assays with HTRF - Videos
This leaflet presents the analytical performances of each assay - Brochures
Get the brochure about technology comparison. - Brochures