Human CXCL10 (IP-10) kit
No-wash kit to quantify released Human CXCL10 (IP-10)
The HTRF human IFN alpha kit is designed for the quantification of human IFN alpha release in cell supernatant.
Mainly produced by leukocytes, IFN alpha is involved in innate immunity response to viral infection. IFN alpha activates several intracellular pathways in target cells, which explains its pleiotropic effects on cell differentiation, growth, and immunoregulation.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IFN-α AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IFN alpha analysis.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers &protocol.|
|LOD &LOQ (in Diluent)||11 pg/mL &36 pg/mL|
|Calibration||NIBSC (94/784) value (IU/mL) = 0,9 x HTRF hIFNa HTRF|
|Range||36 4,000 pg/mL|
|Time to result||2h at RT|
|Sample||Mean [IFNa] (pg/mL)||CV|
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
|Sample||[IFNa] (pg/mL)||Mean (delta R)||CV|
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
In collaboration with CNRS - Scientific Presentations
In collaboration with Molecular Devices - Application Notes
In collaboration with Blood assay solution - Application Notes
Perform and optimize cytokine assays on PBMC - Technical Notes
A fun video introducing you to cytokines assays with HTRF - Videos
This leaflet presents the analytical performances of each assay - Brochures
Get the brochure about technology comparison. - Brochures
Data about HTRF IFNb correlation with gene reporter assay and ELISA - Application Notes