This kit detects the accumulation of inositol monophosphate, a metabolite produced following phospholipase C activation and used as a proxy of Gq activation
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  • Ease-of-use
  • Low false negative
  • Highly specific
  • Accurate pharmacology
This kit detects the accumulation of inositol monophosphate, a metabolite produced following phospholipase C activation and used as a proxy of Gq activation
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Overview

Cisbio IP-One assays detect the accumulation of inositol monophosphate, a stable downstream metabolite of IP3 induced by activation of a phospholipase C (PLC) cascade. This kit allows direct characterization of all types of compounds acting on Gq-coupled receptors in either adherent or suspension cells.

From high-throughput screening (HTS) to structure-activity relationships (SAR), IP-One has been shown to offer superior benefits over traditional assays

Assay principle

The kit is based on a competitive format involving a specific antibody labeled with cryptate (donor) and IP1 coupled to d2 (acceptor). Native IP1 produced by cells competes with d2-labeled IP1 for binding to monoclonal anti-IP1 Tb2+ cryptate. LiCl is added to the cell stimulation buffer, causing IP1 to accumulate upon receptor activation.

Assay protocol

The IP-One assay features a streamlined protocol with just two incubation steps: Cell stimulation by the ligand or target compounds IP1 detection using HTRF reagentsThis protocol requires only a single, one-hour incubation period following cell stimulation.

Specificity of IP-One Gq assay

IP-One ensures signal specificity, measuring only IP-1, an analyte that is solely produced by the action of the phospholipase enzyme (PLC) and mediated by GPCRs.

In contrast, calcium responses are complex in nature. Calcium release is frequently triggered by non-G protein mechanisms, including Ca2+ permeable channels, calcium pumps, calcium transporters.

Standard curve characteristics

Assay Window, sensitivity, reproducibility and specificity : IP-One makes no concession !
Experience on HTRF technology in public-private collaborations

Neogenius Pharma - Scientific Presentations

Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery

In collaboration with Bayer - Scientific Presentations

Structure, function and pharmacology of G protein-coupled receptors: State-of-the-art and future challenges

In collaboration with the Faculty of health and medical sciences Denmark - Scientific Presentations

HTRF assays of IP1, cAMP and pERK pathways employed to study biased signaling of the calcium-sensing receptor

In collaboration with the Faculty of health and medical sciences Denmark - Scientific Presentations

HTRF a versatil approach for 7TM drug discovery

In collaboration with MRC - Scientific Presentations

Case Study: HTS of GPR-54 with IP-One Assay

In collaboration with Harvard Center for Neurodegeneration and Repair - Scientific Presentations

cAMP Fresh and Frozen Cell Usage

In collaboration with Bristol Myers Squibb - Scientific Presentations

IP-One & cAMP HTRF Functional Cell-based Screening

In collaboration with Schering AG - Scientific Presentations

GPCR research from A to Z

HTRF products and supporting scientific content to investifgate GPCRs - Flyers

A comparison of IP-One and FLIPR (calcium) assays

Correlation of 30 known cholinergic antagonists - Technical Notes

A comparison of IP-One and FLIPR (calcium) assays

IC50 & ranking of a selection anti-histaminic blockbuster. - Technical Notes

HTRF assays for Oncology and Inflammation

Signaling in the immune syqtem - Brochures

Characterisation of Inositol Phosphate & cAMP Signalling from GPCRs using HTRF

In collaboration with College of Science, King Saud University, University of Western Australia - Posters

HTRF® technology on the SpectraMax® i3 Multi-Mode Platform

In collaboration with Mollecular Devices - Posters

Differentiation of partial and full GPR40 agonists for the treatment of type 2diabetes

In collaboration with Jansen research & development - Posters

Ultimate guide to master Gq assays

Optimizing IP-One assays has never been easier - Guides

Understanding GPCRs is the key to improved DD

In collaboration with GEN - Guides

HTRF IP-One assay: a robust and stable platform for high throughput screening of Gaq-coupled GPCRs

Overcome calcium assay signal's transient nature in Gq studies - Technical Notes

Product Insert IP-One Gq / 62IPAPEB-62IPAPEC-62IPAPEJ

62IPAPEB-62IPAPEC-62IPAPEJ - Product Insert

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

Safety Data Sheet IP-One Gq / 62IPAPEB

62IPAPEB - Safety Data Sheet

Safety Data Sheet IP-One Gq / 62IPAPEC

62IPAPEC - Safety Data Sheet

Safety Data Sheet IP-One Gq / 62IPAPEJ

62IPAPEJ - Safety Data Sheet

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