PAb Anti-phospho ATF2-Eu cryptate enables the rapid detection of ATF2 phosphorylation on Thr69/71 in a biochemical kinase assay format.
See more
  • No-wash
  • Ease-of-use
  • High affinity
PAb Anti-phospho ATF2-Eu cryptate enables the rapid detection of ATF2 phosphorylation on Thr69/71 in a biochemical kinase assay format.
-
In stock

Overview

The Anti-phospho ATF2-Eu cryptate is a labeled polyclonal antibody enabling the detection of ATF2 when singly phosphorylated at Ser 71 or dually phosphorylated at Thr69/71. It does not detect the non-phosphorylated ATF2. This allows the construction of a highly specific biochemical kinase assay. Any kind of substrate can be used, from small synthetic peptides to natural substrates, meaning high assay detection versatility. As HTRF tolerates extremely high ATP concentrations, an assay can be performed at physiological ATP concentrations.

Benefits

  • NO LIMITATION IN ATP CONCENTRATIONS
  • ANY KIND OF SUBSTRATES
  • PHOSHO-ATF2 (THR71) SPECIFIC

Assay principle

HTRF kinase assays typically use a combination of a Europium-cryptate labeled anti-phosphoresidue antibody, SA-XL665 and a biotinylated* substrate. The signal is proportional to the concentration of phospho-residues. *Alternatively, other tags such as 6HIS, GST, c-myc, DNP, or FLAG may be used to label the kinase substrate.

Assay protocol

HTRF kinase assays have two basic phases: 1. Enzymatic step: biotinylated substrate is incubated with the kinase of interest and with the compounds in presence of co-factors. The reaction starts with the addition of ATP. 2. Detection step: The phosphorylated biotinylated substrate is then detected by the addition of Streptavidin-XL665 and PAb Anti-phospho ATF2-Eu cryptate , prepared in a buffer containing EDTA to stop the enzymatic reaction.
Open R&D: Sanofi Access Platform

In collaboration with Sanofi - Scientific Presentations

Experience the freedom of building your own assay

HTRF kinase activity toolbox reagents - Flyers

Product Insert A-P ATF2-Eu / 61P12KAE-61P12KAZ-61P12KAY

61P12KAE-61P12KAZ-61P12KAY - Product Insert

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

The essential white paper to expand your knowledge on kinases

Learn about cutting edge kinase research in this free white paper - Guides

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

Safety Data Sheet A-P ATF2-Eu / 61P12KAE

61P12KAE - Safety Data Sheet

Safety Data Sheet A-P ATF2-Eu / 61P12KAY

61P12KAY - Safety Data Sheet

Safety Data Sheet A-P ATF2-Eu / 61P12KAZ

61P12KAZ - Safety Data Sheet

tab6

Latest news