The Human CXCL1 (GRO alpha) kit is designed for the quantification of human CXCL1 release in cell supernatant
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  • No-wash
  • Low sample consumption
  • Under 2h bench time
The Human CXCL1 (GRO alpha) kit is designed for the quantification of human CXCL1 release in cell supernatant
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In stock

Overview

Also called GRO alpha, KC NAP-3 (neutrophil activating protein 3), or MGSA-alpha (melanoma growth stimulating activity alpha), CXCL1 is a member of the CXC chemokine family. Mainly expressed by macrophages, CXCL1 attracts neutrophils and is involved in angiogenesis, inflammation, and tumorigenesis.

Benefits

  • SCALABLE FROM 96 TO 1536WELLS
  • PANEL QUANTIFICATION

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your CXCL1 analysis.

Technical specifications of human CXCL1 kit

Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent) 13 pg/mL &47 pg/mL
Range 47 – 4,000 pg/mL
Time to result 2h at 4°C
Species Human only

Intra and inter assay

Intra-assay (n=24)

Sample Mean [CXCL1] (pg/mL) CV
1 150 8%
2 1500 6%
3 3000 9%
Mean CV 8%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample [CXCL1] (pg/mL) Mean (delta R) CV
1 212 350 13%
2 686 934 2%
3 2222 1904 1%
Mean CV 5%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

HTRF Cytokine cell-based assays

Monitor immune regulation the simple way - Flyers

HTRF assays for Oncology and Inflammation

Signaling in the immune system - Brochures

Normalize HTRF cytokine assays with viability assay on Spectramax I3X-Minimax

In collaboration with Molecular Devices - Application Notes

HTRF measurement of cytokine release from fresh blood samples

In collaboration with Blood assay solution - Application Notes

Cytokine secretion profile on PBMCs

One sample to measure them all - Posters

Guidelines from PBMC isolation to cytokine assay optimisation

Perform and optimize cytokine assays on PBMC - Technical Notes

How to freeze PBMC

Validated protocol - Technical Notes

How to measure multiple cytokines from 1 sample

What to expect at the bench - Videos

Product Insert CXCL1 (h) Kit / 62HCXC1PEG-62HCXC1PEH

62HCXC1PEG-62HCXC1PEH - Product Insert

HTRF makes cytokine detection easy

A fun video introducing you to cytokines assays with HTRF - Videos

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

Cytokine assays: a guide to success with HTRF

Setting up a successful cytokine assay - Guides

Portfolio of HTRF cytokine assays

This leaflet presents the analytical performances of each assay - Brochures

How HTRF compares to Western Blot and ELISA

Get the brochure about technology comparison. - Brochures

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