Human CCL20 (MIP3 alpha) kit
No-wash kit to quantify released Human CCL20 (MIP3 alpha)
The HTRF human IL17 kit is designed for the quantification of human IL17 release in cell supernatant.
IL17 is a pro-inflammatory cytokine induced by IL23 and produced by helper T cells. IL17 is part of the response to fungi and bacteria, and promotes the release of other pro-inflammatory cytokines and chemokines. Due to its role as a mediator of inflammatory responses, IL17 has been linked to allergic responses and autoimmune diseases like asthma, psoriasis, or IBD (inflammatory bowel disease). In immuno-oncology, IL17 is the main cytokine released by differentiated TH17 cells.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable the detection of low levels of analytes in serum or plasma. Check out the Human IL-17 AlphaLISA kit’s analytical performances for more information or learn more about AlphaLISA. When assaying, always follow the recommended protocol and avoid highly haemolyzed samples.
The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.
To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.
Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL17 analysis.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers &protocol.|
|LOD &LOQ (in Diluent)||7 pg/mL &33 pg/mL|
|Range||33 10,000 pg/mL|
|Time to result||Overnight at RT|
|Calibration||NIBSC (01/420) value (IU/mL) = 0,02 x HTRF hIL17 value (pg/mL)|
|Sample||Mean [IL17] (pg/mL)||CV|
Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.
|Sample||[IL17] (pg/mL)||Mean (delta R)||CV|
Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.
In collaboration with CNRS - Scientific Presentations
In collaboration with Novartis - Scientific Presentations
In collaboration with Molecular Devices - Application Notes
In collaboration with Blood assay solution - Application Notes
Perform and optimize cytokine assays on PBMC - Technical Notes
A fun video introducing you to cytokines assays with HTRF - Videos
This leaflet presents the analytical performances of each assay - Brochures
Get the brochure about technology comparison. - Brochures
Learn how peer researchers monitor cytokines with HTRF - Application Notes
A step by step protocol for Th17 cells differentiation from naive CD4 mouse cells - Technical Notes