This immune checkpoint assay is designed to identify human CD47/SIRP alpha inhibitors.
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  • All inclusive kit
  • Scalable from 96 to 1536 format
  • Validated with reference Ab
This immune checkpoint assay is designed to identify human CD47/SIRP alpha inhibitors.
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In stock

Overview

Signal-regulatory protein alpha (SIRP-alpha), also known as CD172a, is a cell surface protein expressed mainly by myeloid cells such as macrophages. Its receptor, CD47, is ubiquitously expressed on the surface of all cells and has been found to be overexpressed in some cancers. CD47 binding to SIRP-alpha delivers a “Do Not Eat Me” signal to macrophages. In the case of a tumor, this signaling aids in tumor evasion of the immune system. Inhibitors of this interaction hold great promise for cancer immunotherapy.

Assay principle

This CD47/SIRP alpha kit includes human recombinant immune checkpoint partners tagged for detection (CD47 and SIRP alpha), a reaction buffer, and labelled anti-tag antibodies for HTRF detection. Without treatment, CD47 binds to SIRP alpha, and the binding of each detection antibody to its tag target generates an HTRF signal. In the presence of a compound or an anti-human blocking antibody, the CD47/SIRP alpha interaction is disrupted and the HTRF signal decreases.

Inhibitory effect of blocking mouse reference Ab

The inhibitory effects of mouse anti-human CD47 were tested at 2nM CD47 and 1 nM SIRPa. An assay window around 4 was obtained.
CD47/SIRPa binding assay

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