The Human IL23 kit is designed for the quantification of human IL23 release in cell supernatant.
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  • No-wash
  • Highly specific
  • Under 2h bench time
The Human IL23 kit is designed for the quantification of human IL23 release in cell supernatant.
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In stock

Overview

IL23 is part of the response against infection as it upregulates the expression of MMP9 (matrix metalloprotease 9), increases angiogenesis, and reduces CD8 T cell infiltration into tumors. In oncology and immuno-oncology, IL23 has been studied for its effects on T cell infiltration and its promotion on TH17 cell development. IL23 is a heterodimeric cytokine composed of p19 and p40 subunits, the latter being shared with IL12. Cisbio's Human IL23 assay is highly specific for IL23 and does not cross react with IL12.

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IL23 analysis.

Technical specifications of human IL23 kit

Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent) 30 pg/mL &92 pg/mL
Range 92 – 30,000 pg/mL
Time to result 3h at 4°C
Species Human only

Intra and inter assay

Intra-assay (n=24)

Sample Mean [IL23] (pg/mL) CV
1 200 10%
2 10,000 2%
3 20,000 2%
Mean CV 5%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample[IL23] (pg/mL)
Mean (delta R)CV
1307
259
12%
21920
1534
7%
312,000
8601
3%


Mean CV7%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample

HTRF Cytokine cell-based assays

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In collaboration with Molecular Devices - Application Notes

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In collaboration with Blood assay solution - Application Notes

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One sample to measure them all - Posters

Guidelines from PBMC isolation to cytokine assay optimisation

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How to freeze PBMC

Validated protocol - Technical Notes

How to measure multiple cytokines from 1 sample

What to expect at the bench - Videos

Product Insert IL23 (h) Kit / 62HIL23PEG-62HIL23PEH

62HIL23PEG-62HIL23PEH - Product Insert

HTRF makes cytokine detection easy

A fun video introducing you to cytokines assays with HTRF - Videos

Cisbio Product Catalog 2019

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

Cytokine assays: a guide to success with HTRF

Setting up a successful cytokine assay - Guides

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