The Mouse IFN gamma kit is designed for the quantification of mouse IFN gamma release in cell supernatant.
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  • No-wash
  • Low sample consumption
  • Under 2h bench time
The Mouse IFN gamma kit is designed for the quantification of mouse IFN gamma release in cell supernatant.
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In stock

Overview

IFN gamma is a pro-inflammatory cytokine secreted by T and NK cells in response to viral infection and other inflammatory conditions. IFN gamma plays a major role in innate and adaptive immunity by promoting T cell differentiation into TH1 and the development of Treg, but also by inhibiting T cell differentiation into TH17. Its anti-tumor properties have been used in the treatment of osteoporosis and it is of great interest in immuno-oncology, where it improves survival in patients with bladder or ovarian carcinoma.

Assay principle

Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Cisbio low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536-well format by simply resizing each addition volume proportionally.

Assay data analysis

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

To fully understand how to deal with HTRF data processing and also 4PL 1/y² fitting, please visit this page.

Cisbio also worked with Myassays.com to help you in your data analysis. By clicking on this link, you’ll be able to access a free online software to run your IFN gamma analysis.

Technical specifications of mouse IFN gamma kit

Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers &protocol.
LOD &LOQ (in Diluent) 23 pg/mL &34 pg/mL
Range 34 – 3,000 pg/mL
Time to result 2h at RT
Species Mouse only

Intra and inter assay

Intra-assay (n=24)

SampleMean [IFNg] (pg/mL)CV
18011%
213752%
327502%

Mean CV5%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample[IFNg] (pg/mL)Mean (delta R)CV
1942135%
237586810%
31500350213%


Mean CV9%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

HTRF Cytokine cell-based assays

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Signaling in the immune syqtem - Brochures

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In collaboration with Molecular Devices - Application Notes

HTRF® measurement of cytokine release from fresh blood samples

In collaboration with Blood assay solution - Application Notes

Cytokine secretion profile on PBMCs

One sample to measure them all - Posters

Guidelines from PBMC isolation to cytokine assay optimisation

Perform and optimize cytokine assays on PBMC - Technical Notes

How to freeze PBMC

Validated protocol - Technical Notes

How to measure multiple cytokines from 1 sample

What to expect at the bench - Videos

Product Insert IFN g (m) Kit / 62MIFNGPEG-62MIFNGPEH

62MIFNGPEG-62MIFNGPEH - Product Insert

HTRF makes cytokine detection easy

A fun video introducing you to cytokines assays with HTRF - Videos

HTRF Product Catalog

All your HTRF assays in one document! - Catalog

A guide to Homogeneous Time Resolved Fluorescence

General principles of HTRF - Guides

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