SNAP tag D2 Dopamine type 2R cloned plasmid
Tag-lite plasmid cloned with a SNAP tag D2 Dopamine type 2 receptor
Dopamine D2 and D3aR fluorescent probe HTRF®
This Dopamine D2 receptor red antagonist is a spiperone derivative labeled with a red emitting HTRF fluorescent probe, to be used in the Tag-lite system.
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Non-radioactive
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No-wash
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Cell-based
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Equilibrium & Kinetic binding compatible
Overview
Tag-lite receptor binding assays use either a green or a red fluorescent ligand to function. This Dopamine D2 receptor red antagonist is a spiperone derivative labeled with a red emitting HTRF fluorescent probe.
This ligand is best suited for use in the Dopamine D2 and Dopamine D3a receptor binding assays.
Benefits
- FLUORESCENTLY LABELED LIGAND
- HIGHLY STABLE
- VALIDATED IN BINDING
Assay principle
Running a receptor binding assay using Tag-lite is as easy as it can get. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.
Saturation binding (KD)
A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.
Watch this video explaining how to run a saturation binding assay using Tag-lite.
Competitive binding (KI)
A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.
Watch this video explaining how to run a competitive binding assay using Tag-lite.
Evaluation of a Tag-lite binding assay for a class B receptor
In collaboration with Boehringer Ingelheim - Scientific Presentations
Ultra HTS at Bayer: use of IP-One and Tag-lite assays in GPCR drug discovery
In collaboration with Bayer - Scientific Presentations
The easy way to approach biology without radioactivity - Flyers
In collaboration with Molecular Devices - Posters
Challenge the limits of binding kinetics studies - Application Notes
Understanding GPCRs is the key to improved DD
In collaboration with GEN - Guides
Tag-lite, the easiest solution for studying GPCR-ligands interactions
The gold standard technology for receptor binding studies - Videos
Product Insert DopD2-R red antago / L0002RED
L0002RED - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Webinar | Association Rate Constant: The Unsung Hero of Drug Discovery Kinetics
Available On-demand - Videos
Easy method for kinetic binding determination
How to revolutionize your kinetic binding demonstration with HTRF kinase binding platform assays - Application Notes
A comprehensive overview of pharmacology's main ligands - Guides
GPCRs: the pathway to Discovery
Complete solutions for GPCR Drug Discovery - Brochures
Safety Data Sheet DopD2-R red antago / L0002RED
L0002RED - Safety Data Sheet
Safety Data Sheet (DEU) DopD2-R red antago / L0002RED
L0002RED - Safety Data Sheet
Safety Data Sheet (ELL) DopD2-R red antago / L0002RED
L0002RED - Safety Data Sheet
Safety Data Sheet (FRA-FR) DopD2-R red antago / L0002RED
L0002RED - Safety Data Sheet
Safety Data Sheet (ITA) DopD2-R red antago / L0002RED
L0002RED - Safety Data Sheet
Safety Data Sheet (SPA) DopD2-R red antago / L0002RED
L0002RED - Safety Data Sheet
Safety Data Sheet (ENG-GB) DopD2-R red antago / L0002RED
L0002RED - Safety Data Sheet
Safety Data Sheet (ENG-US) DopD2-R red antago / L0002RED
L0002RED - Safety Data Sheet
Batch Information DopD2-R red antago / 20220501
L0002RED Batch 07A - Batch Information
Batch Information DopD2-R red antago / 20231201
L0002RED Batch 07A - Batch Information
Batch Information DopD2-R red antago / 20240301
L0002RED Batch 07B - Batch Information
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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