Total H3 Cellular assay for studying methyltransferases and demethylases
This cellular assay is for the direct detection of endogenous levels of Total H3 (histone 3) in cells. It has been developed with optimized reagents and protocols. HTRF® is a flexible assay format enabling you to use an assay for a variety of applications, without having to compromise on sensitivity. The Total H3 assay can be used for adherent cells or suspension cells, primary or secondary screening, inhibitor studies and normalization studies of both methyltransferases and demethylases. The total H3 kit has been validated with Cisbio’s EPIgeneous H3K27ME3 and H3K36ME2 kits for normalization studies.