High Throughput Tag-Lite Cell-based Functional and Surface Binding Assays on the SpectraMax Plate Reader Platform

Oksana Sirenko, Marie-Laure Lebreton, Delphine Jaga, Michael Katzlinger, Laurence Monnet, Caroline Cardonnel, H. Roger Tang, Cathy Olson, Francois Degorce, Evan F. Cromwell

2011

Molecular Devices, Inc. Sunnyvale, CA. Cisbio Bioassays, Codolet, France.

G-protein coupled receptors (GPCR) are the largest class of cell-surface receptors and are targets for almost 40% of existing drugs. Lead discovery, testing the efficacy of prospective drugs (in the area of cardiovascular diseases and other fields), and understanding of mechanism of action of drug candidates requires assays that can measure the binding of ligands to the receptors, receptor oligomerization, and/or internalization. Accordingly, there is a real need for robust and sensitive assays of this type that are suitable for high throughput screening. The Tag-lite® cellular screening platform was designed to increase the flexibility of cell-surface receptor research. This platform, which combines HTRF® and SNAP-tag® technologies, is ideal for primary and secondary screening and can be applied to a variety of assay formats for pharmacological characterization and development of therapeutic antibodies. Here we show results from use of this assay platform on the only user upgradable plate reader – the SpectraMax® Paradigm system, and the widely used M5e plate reader.

HTRF microplate readers