Cisbio Bioassays, Codolet, France
Cisbio offers a comprehensive line of HTRF phospho- and total protein assays to investigate cell signaling pathways. These versatile, homogeneous assays are suitable for a variety of sample types, including primary cell lines and tumor xenografts. Each HTRF assay includes a common and essential step that relies on the ability of the lysis buffer to release the protein of interest. This protein, either phosphorylated or not, is then detected by an HTRF sandwich immunoassay.
This application note compares the use of Cisbio’s HTRF phospho-total lysis buffer with commonly used RIPA lysis buffers for the analysis of cell signaling pathways in different types of samples. The data demonstrate that HTRF phospho-total lysis buffer is as efficient as RIPA lysis buffers to perform protein extraction and is compatible with both simple and more complex biological samples. Moreover, the HTRF phospho-total lysis buffer delivers higher quality HTRF results than RIPA lysis buffers. Finally the HTRF phospho-total Cisbio lysis buffer is not only suitable for HTRF, but also for other analytical methods such as Western blot and ELISA.