Laurence Monnet, Cathy Olsen, and Marie-Laure Lebreton
Molecular Devices, Sunnyvale, USA. Cisbio Bioassays, Codolet, France.
HTRF® assays have developed over the years to encompass the areas of G protein‐coupled receptors, kinases and cell signaling, epigenetics and biomarkers. A wide variety of toolbox reagents address assays including protein‐protein interactions, nuclear receptor assays, receptor dimerization, ligand binding, receptor internalization and enzyme assays. HTRF technology requires specific detection conditions. Timeresolved detection of two different wavelengths compensates for compound interference and sample quenching and also minimizes background fluorescence detection. A plate reader’s optics, energy source, and detectors are critical for measuring HTRF output. Instrument set‐up also has a significant impact on detection efficiency. Cisbio’s certification program guarantees that readers bearing the HTRF sticker meet the physical specifications for optimal HTRF readout. The new SpectraMax® i3 Multi‐Mode Platform uses HTRF detection cartridges with optimized components to meet and exceed the specifications required to perform the full range of HTRF assays