HTRF® measurement of cytokine release from fresh blood samples

Cisbio, Codolet, France

Cisbio offers a comprehensive line of HTRF cytokine assays to investigate the functional response of immune cells. These versatile, homogeneous assays are suitable for a variety of sample types, such as cell culture supernatants from immortalized cell lines or more physiological cell models such as Peripheral Blood Mononuclear Cells (PBMCs).
This technical note describes how to purify PBMC from a buffy coat or whole blood sample using Ficoll gradient, and introduces the optimization steps needed for an appropriate PBCM cytokine quantification using HTRF assays.