CCL5, also known as RANTES (regulated upon activation, normal T-cell expressed and secreted), is a chemokine mainly secreted by T lymphocytes, macrophages, platelets, synovial fibroblasts, tubular epithelium and certain types of tumor cells. CCL5 plays an important role on inflammatory sites by recruiting T cells, macrophages, eosinophils, and basophils. When secreted with IL-2 and IFN-g, CCL5 also induces the activation and proliferation of natural killer cells.
The functions of CCL5 in a tumor environment is still unclear, as CCL5 can act to induce an immune response against the tumor but is also associated with cancer progression and metastasis. In infectious diseases, CCL5 has been described as inhibiting HIV entry into target cells.
Cisbio's Human CCL5 kit is a homogeneous, add and read assay to measure CCL5 secretion in cell supernatant. It offers a fast and efficient alternative to ELISA
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers & protocol|
|LOD & LOQ (in Diluent)||7 pg/mL & 18 pg/mL|
|Range||18 – 2,000 pg/mL|
|Time to result||ON @ RT|
|Calibration||NIBSC (92/520) value (IU/mL) = 0,015 x HTRF hCCL5 value (pg/mL)|