Human G-CSF Assay Kit

Also known as CSF3 (colony-stimulating factor 3), G-CSF is a hematopoietic cytokine. It acts on precursor cells in the bone marrow to initiate their proliferation and differentiation into mature granulocytes, on hematopoietic stem cells to induce their mobilization in the blood stream, and on neurons to promote neurogenesis and block apoptosis.
In myocardial infarction or ischemia, G-CSF promotes T cells' immune tolerance and tissue recovery.
The absence of a functional G-CSF receptor is associated with congenital neutropenia
Cisbio's Human G-CSF kit is a homogeneous, add and read assay to measure G-CSF secretion in supernatant. It offers a fast, efficient alternative to ELISA.

Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers & protocol.
LOD & LOQ (in Diluent) 27 pg/mL & 47 pg/mL
Range 47 – 4,500 pg/mL
Time to result Overnight at RT
Calibration NIBSC (09/136) value (IU/mL) = 0,07 x HTRF hGCSF HTRF
Species Human only
Download Insider tips for PBMC isolation Technical Note
Download cytokine release from fresh blood samples application notes

Assay principle

The assay protocol, using a Cisbio 96-well low volume or a 384-well small volume white plate (20 µL final), is described below. Cell supernatant, sample or standard are dispensed directly into the assay plate for the detection by HTRF® reagents. The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in 96 to 1536-well plates by simply resizing each addition volume proportionally.

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

Add

Cytokines assay principle add

Read

Cytokines assay principle read

(1) Assay data analysis:

  • "Guidelines for data reduction" button will show you how dealing with HTRF data processing and 4PL 1/y2 fitting.
  • "MyAssays" button will direct you to a free online software able to run GCSF analysis.

 

How to use HTRF cytokine assays

How to measure multiple cytokines from 1 sample

Analytical performance

Precision

Intra-assay (n=24)

Sample Mean [G-CSF] (pg/mL) CV
1 47 18%
2 2036 7%
3 3780 6%
  Mean CV 10%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample [G-CSF] (pg/mL) Mean (delta R) CV
1 87 222 13%
2 423 883 8%
3 2045 3161 6%
    Mean CV 9%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Human G-CSF kit - 500 tests62HGCSFPEG
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Human G-CSF kit - 10,000 tests62HGCSFPEH
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Companion products

DescriptionCat. noProduct insertMSDS
Human G-CSF kit - 3 Standards62HGCSFCD3
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Human G-CSF kit - Standard62HGCSFCDA
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Documents