Also known as CSF2 (colony-stimulating factor 2), GM-CSF (granulocyte-macrophage colony stimulating factor) is a hematopoietic growth factor and an immune modulator. Under immune stimuli, it is produced by T lymphocytes, macrophages, endothelial cells and fibroblasts.
It acts on precursor cells in the bone marrow to initiate their proliferation and differentiation into mature granulocytes and macrophages. GM-CSF stimulates multipotent progenitor cells.
Cisbio's human GM-CSF kit is a homogeneous, add and read assay to measure G-CSF secretion in cell supernatant. It offers a fast and efficient alternative to ELISA.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers & protocol|
|LOD & LOQ (in diluent)||29 pg/mL & 54 pg/mL|
|Range||54 – 8,500 pg/mL|
|Time to result||3h @ RT|
|Calibration||NIBSC (88/646) value (IU/mL) = 0.01 x HTRF hGM-CSF value (pg/mL)|
|Species||Human and cynomolgus|