Human Heme Oxygenase Assay Kit

HTRF® assay kit for detecting human heme oxygenase in cell lysates

Based on our TR-FRET homogeneous and robust HTRF® technology, the human HO-1 sandwich immunoassay kit is designed for the rapid detection of endogeneous human heme oxygenase protein in cell lysates. Using a streamlined no-wash protocol, this assay gives enhanced throughput capabilities compared to conventional technologies like ELISA. Its simple and robust procedure is suitable for miniaturization and enables work with sample volumes as low as 5 µL, saving precious biological samples. With this assay, human heme oxygenase can be directly detected in your cell type of interest, including primary cells.

Assay principle

Human heme oxygenase is measured using sandwich immunoassays involving two specific human heme oxygenase antibodies, respectively labelled with Europium cryptate (donor) and d2 (Acceptor). The intensity of the signal is proportional to the concentration of the heme oxygenase present in the sample.

The human heme oxygenase assay protocol is described here. Cells are plated, stimulated and next lysed in the same 96-well culture plate. Lysates are then transferred to the assay plate for the detection of human heme oxygenase by HTRF® reagents. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Human Heme Oxygenase kit - 500 tests63ADK044PEG
Human Heme Oxygenase kit - 10,000 tests63ADK044PEH

Companion products

DescriptionCat. noProduct insertMSDS
Human Heme Oxygenase kit - control63ADK044TDA