IFNγ is a pro-inflammatory cytokine secreted by T cells and NK cells in response to viral infection and other inflammatory conditions. It induces the expression of class II MHC, macrophage activation, and the Immunoglobulin switch in B cells. IFNγ plays a major role in innate and adaptive immunity by promoting T cell differentiation into TH1 and the development of Treg, but also by inhibiting T cell differentiation into TH17. Its anti-tumor properties have been used in the treatment of osteoporosis and it is of great interest in immuno-oncology where it improves survival in patients with bladder or ovarian carcinoma.
Cisbio's Human IFNγ kit is a homogeneous, add and read assay to measure IFNγ secretion in supernatant. It offers a fast, efficient alternative to ELISA.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers & protocol.|
|LOD & LOQ (in Diluent)||14 pg/mL & 21 pg/mL|
|Range||21 – 4,000 pg/mL|
|Time to result||Overnight at RT|
|Calibration||NIBSC (82/587) value (IU/mL) = 0,019 x HTRF hIFNγ value (pg/mL)|