Also known as hematopoietin 1, IL1α is a pro-inflammatory cytokine mainly produced by activated macrophages, epithelial and endothelial cells. Its main role is the maintenance and protection of the skin's barrier function. In response to infection, IL1α stimulates fibroblast proliferation, increases blood neutrophils, and activates lymphocyte proliferation.
Cisbio's Human IL1α kit is a homogeneous, add and read assay to measure IL1α secretion in supernatant. It offers a fast, efficient alternative to ELISA.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers & protocol.|
|LOD & LOQ (in Diluent)||8 pg/mL & 20.5 pg/mL|
|Range||20.5 – 5,000 pg/mL|
|Time to result||2h at RT|
|Calibration||NIBSC (86/632) value (IU/mL) = 10 x HTRF hIL1α value (pg/mL)|