Also called IGIF (interferon gamma inducing factor), IL18 is a pro-inflammatory cytokine secreted mainly by macrophages. Its biological activity is close to that of IL12, and it can enhance NK cell activity as well as induce IFNg secretion by PBMC.
An important role of IL18 is in eliminating VEGF from the eye, thus protecting the retina. It is used together with anti-VEGF antibodies in the treatment of AMD (age-related macular degeneration).
As well as this physiological role, IL18 dysregulation leads to severe inflammatory reactions.
Cisbio's Human IL18 kit is a homogeneous, add and read assay to measure IL18 secretion in supernatant. It offers a fast, efficient alternative to ELISA.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers & protocol.|
|LOD & LOQ (in Diluent)||18 pg/mL & 43 pg/mL|
|Range||43 – 8,000 pg/mL|
|Time to result||1h at RT|
|Calibration||NIBSC (03/200) value (IU/mL) = 5 x HTRF hIL18 value (pg/mL)|