Human IL23 Assay Kit

IL23 is part of the response against infection, as it upregulates the expression of MMP9 (matrix metalloprotease 9), increases angiogenesis and reduces CD8 T cell infiltration into tumors.
In oncology and immuno-oncology, IL23 has been studied for its effects on T cell infiltration and its promotion on TH17 cell development.
IL23 is a heterodimeric cytokine composed of p19 and p40 subunits, the latter being shared with IL12. Cisbio's Human IL23 assay is highly specific for IL23 and does not cross react with IL12.
The kit is a homogeneous, add and read assay to measure IL23 secretion in supernatant. It offers a fast, efficient alternative to ELISA.

Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers & protocol.
LOD & LOQ (in Diluent) 30 pg/mL & 92 pg/mL
Range 92 – 30,000 pg/mL
Time to result 3h at 4°C
Species Human only
Download Insider tips for PBMC isolation Technical Note
Download cytokine release from fresh blood samples application notes

Assay principle

The assay protocol, using a Cisbio 96-well low volume or a 384-well small volume white plate (20 µL final), is described below. Cell supernatant, sample or standard are dispensed directly into the assay plate for the detection by HTRF® reagents. The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in 96 to 1536-well plates by simply resizing each addition volume proportionally.

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

Add

Cytokines assay principle add

Read

Cytokines assay principle read

(1) Assay data analysis:

  • "Guidelines for data reduction" button will show you how dealing with HTRF data processing and 4PL 1/y2 fitting.
  • "MyAssays" button will direct you to a free online software able to run hIL23 analysis.

 

How to use HTRF cytokine assays

How to measure multiple cytokines from 1 sample

Analytical performance

Precision

Intra-assay (n=24)

Sample Mean [IL23] (pg/mL) CV
1 200 10%
2 10,000 2%
3 20,000 2%
  Mean CV 5%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample [IL23] (pg/mL) Mean (delta R) CV
1 307 259 12%
2 1920 1534 7%
3 12,000 8601 3%
    Mean CV 7%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Human IL23 kit - 500 tests62HIL23PEG
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Human IL23 kit - 10,000 tests62HIL23PEH
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Companion products

DescriptionCat. noProduct insertMSDS
Human IL23 kit - 3 Standards62HIL23CD3
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Human IL23 kit - Standard62HIL23CDA
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Documents