IL4 is expressed by T cells, NK cells and mast cells, and is an important regulator of humoral and adaptative immunity. It stimulates activated B cells, and induces their differentiation into plasma cells and their class switch to immunoglobulin E, as well as promoting class II MHC expression.
In immuno-oncology, IL4 induces the differentiation of naïve T cells into TH2 cells, which in turn produce IL4 and thus create a positive feedback loop.
IL4 is also involved in the development of airway inflammation and allergic asthma.
Cisbio's Human IL4 kit is a homogeneous, add and read assay to measure IL4 secretion in supernatant. It offers a fast, efficient alternative to ELISA.
|Sample size||16 µL|
|Final assay volume||20 µL|
|Kit components||Lyophilized standard, frozen detection antibodies, buffers & protocol.|
|LOD & LOQ (in Diluent)||8 pg/mL & 29 pg/mL|
|Range||29 – 4,000 pg/mL|
|Time to result||2h at RT|
|Calibration||NIBSC (88/656) value (IU/mL) = 0,012 x HTRF hIL4 value (pg/mL)|