Human Pro-collagen type 1 Assay Kit

HTRF® assay kit for detecting human pro-collagen type 1 in cell supernatants

Cisbio’s homogeneous and robust HTRF® human pro-collagen type 1 assay kit is designed for the quantitative detection of human pro-collagen type 1 modulation, using cell supernatants, whole cells or recombinant protein extracts. Only a low volume of sample is required, which means precious biological material savings. The simple add & read protocol eliminates all wash steps for a faster analysis and easy miniaturization. The ready-to-use kit contains all the reagents you need and offers enhanced convenience over other immunoassay technologies.

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Assay principle

Human pro-collagen is measured using sandwich immunoassays involving two specific anti-human pro-collagen antibodies, respectively labelled with Europium cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the pro-collagen present in the sample.

The human pro-collagen type 1 assay protocol is described here, using a 384-well small volume white plate (20 µL final). Cell supernatant, sample or standard are dispensed directly into the assay plate for the detection of human pro-collagen by HTRF® reagents. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Pro-collagen Type 1 (human) kit- 500 tests63ADK014PEG
Pro-collagen Type 1 (human) kit- 10,000 tests63ADK014PEH

Companion products

DescriptionCat. noProduct insertMSDS
Diluent 1 - 20 ml62DL1DDD
Pro-collagen Type 1 (human) kit- Standard63ADK014CDA