Human Progranulin Assay Kit

Sandwich immunoassay kit designed for the rapid detection of human progranulin 

Progranulin, also called proepithelin or prostate cancer (PC) cell-derived growth factor, is the precursor protein of granulin. Progranulin is involved in Frontotemporal Lobar Degeneration (FTLD) and Alzheimer's Disease, where it may protect against beta amyloid toxicity. This cell-based HTRF assay ensures quick results (overnight incubation) and is adapted to work with samples as low as 5 µL, saving precious biological material.

Assay principle

HTRF® - the homogeneous sandwich immunoassay

Human Progranulin is measured using sandwich immunoassays involving two specific anti-human Progranulin antibodies, respectively labeled with Terbium cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the Progranulin present in the sample.

Assay Protocol

The simple Progranulin assay protocol, using a 384-well small volume white plate (20 µL final), is described on the right. Cell supernatant, sample or standard are dispensed directly into the assay plate for the detection of human Progranulin by HTRF® reagents. The antibodies labeled with HTRF donor and acceptor may be pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Human Progranulin kit - 500 tests63ADK084PEG
Human Progranulin kit - 10,000 tests63ADK084PEH

Companion products

DescriptionCat. noProduct insertMSDS
Human Progranulin kit - Standard63ADK084CDA