Leukotriene B4 & Leukotriene C4 Assays Kits

Prostaglandins and leukotrienes derive enzymatically from the Arachidonic acid cascade. Cyclooxygenase (Cox-1 or -2) pathways produce prostaglandins, while lipooxygenase pathways synthesize leukotrienes. PGE synthase converts prostaglandin (PG) H2 into PGE2, which is involved in several pathologies such as periodontal disease and promoting tumor cell survival. 5-lipoxygenase catalyses the conversion of Arachidonic acid into 5-HPETE (5-hydroperoxyeicosatetraenoic acid) and then into LTA4. While the addition of glutathione to LTA4 produces LTC4, LTA4-hydrolase converts LTA4 into LTB4. Leukotrienes are very important agents in inflammation pathways, and are involved in asthmatic and allergic reactions.

Assay principle

The assays are competitive immunoassays that uses Eu3+-Cryptate labeled anti-leukotriene. Free leukotrienes from the sample compete with acceptor labeled leukotriene (XL665 for LTB4 and d2 for LTC4) for binding to the Cryptate conjugated anti-leukotriene antibody. The assays can be run in two easy steps, and require only a single incubation following cell stimulation. Assays can also be run with purified enzymes.

Product specifications

  LTB4 LTC4
Incubation 1h at RT 1h at RT
Assay range 0.15 – 166.67 ng/mL 0.1 – 5000 ng/mL
EC50 1.12 ng/mL 2.7 ng/mL
Detection limit 0.15 ng/mL 0.1 ng/mL
Specificity Low cross reactivity with 6-trans 12 epi LTB4 No cross reactivity with LTA4, LTB4

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
LTB4 kit - 500 tests64LB4PEG
LTB4 kit - 10,000 tests64LB4PEH
-
LTC4 kit - 500 tests64LC4PEG
-
LTC4 kit - 10,000 tests64LC4PEH

Companion products

DescriptionCat. noProduct insertMSDS
Diluent 3 - 20 ml62DL3DDD
LTC4 kit standard64LC4C0A

Documents