HTRF® MAP-Tau kit is a rapid sandwich immunoassay for accurate quantification of the microtubule-associated protein Tau in cell lysates. Only a low volume of sample is required, saving biological material. With no washing steps, the simple and robust procedure gives increased throughput compared to ELISAs.
MAP-Tau is measured using sandwich immunoassays involving two specific anti-MAP-Tau antibodies, respectively labelled with Europium cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the MAP-Tau present in the sample.
The MAP-Tau assay protocol is described here. Cells are plated, stimulated and next lysed in the same 96-well culture plate. Lysates are then transferred to the assay plate for the detection of human MAP-Tau by HTRF® reagents. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
| Description | Cat. no | Product insert | MSDS |
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| MAP-Tau kit - 500 tests | 63ADK008PEG |
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| MAP-Tau kit - 10,000 tests | 63ADK008PEH |
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| Description | Cat. no | Product insert | MSDS |
|---|---|---|---|
| MAP-Tau kit - Standard | 63ADK008CDA |
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| Phospho-total protein lysis buffer #1 - 130 mL | 64KL1FDF |
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Cell-based assays for neurosciences find_in_pageCisbio Bioassays, Codolet, France |
Detection of human tau protein aggregation - BMG Labtech Pherastar FSDelphine Jaga, and Franka Maurer - Cisbio, France - BMG LABTECH, Germany | Download |
Truly homogeneous functional assays
FRET (Fluorescence Resonance Energy Transfer) is based on the transfer of energy between two fluorophores – a donor and an acceptor – when in close proximity. Molecular interactions between biomolecules can be assessed by coupling each partner with a fluorescent label and by detecting the level of energy transfer.
