Mouse IL1 beta Assay Kit

IL1β, also known as leukocytic pyrogen or mononuclear cell factor, is a pro-inflammatory cytokine of the IL1 family. IL1β is considered to be a major endogeneous pyrogen and induces prostaglandin synthesis, T cell activation, B cell activation and subsequent antibody production. It is also a known promoter of T cell differentiation into Th17.
Cisbio's Mouse IL1β kit is a homogeneous, add and read assay to measure IL1β secretion in supernatant. It offers a fast, efficient alternative to ELISA.

Sample size 16 µL
Final assay volume 20 µL
Kit components Lyophilized standard, frozen detection antibodies, buffers & protocol.
LOD & LOQ (in Diluent) 14 pg/mL & 46 pg/mL
Range 46 – 4,200 pg/mL
Time to result ON at RT
Calibration NIBSC (93/668) value (IU/mL) = 0,1 x HTRF mIL1β value (pg/mL)
Species Mouse only
Download Insider tips for PBMC isolation Technical Note
Download cytokine release from fresh blood samples application notes

Assay principle

The assay protocol, using a Cisbio 96-well low volume or a 384-well small volume white plate (20 µL final), is described below. Cell supernatant, sample or standard are dispensed directly into the assay plate for the detection by HTRF® reagents. The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run in 96 to 1536-well plates by simply resizing each addition volume proportionally.

The 4 Parameter Logistic (4PL) curve is commonly recommended for fitting an ELISA standard curve. This regression enables the accurate measurement of an unknown sample across a wider range of concentrations than linear analysis, making it ideally suited to the analysis of biological systems like cytokine releases.

Add

Cytokines assay principle add

Read

Cytokines assay principle read

(1) Assay data analysis:

  • "Guidelines for data reduction" button will show you how dealing with HTRF data processing and 4PL 1/y2 fitting.
  • "MyAssays" button will direct you to a free online software able to run IL1β analysis.

 

How to use HTRF cytokine assays

How to measure multiple cytokines from 1 sample

Analytical performance

Precision

Intra-assay (n=24)

Sample Mean [IL1β] (pg/mL) CV
1 100 6%
2 800 2%
3 3200 2%
  Mean CV 3%

Each of the 3 samples was measured 24 times, and % CV was calculated for each sample.

Inter-assay (n=4)

Sample [IL1β] (pg/mL) Mean (delta R) CV
1 170 272 2%
2 612 1023 4%
3 2211 3009 1%
    Mean CV 2%

Each of the samples was measured in 4 different experiments, and % CV was calculated for each sample

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Mouse IL1 beta kit - 500 tests62MIL1BPEG
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Mouse IL1 beta kit - 10,000 tests62MIL1BPEH
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Companion products

DescriptionCat. noProduct insertMSDS
Mouse IL1 beta kit - 3 Standards62MIL1BCD3
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Mouse IL1 beta kit - Standard62MIL1BCDA
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