Cisbio, Codolet, France
HTRF Cell Signaling assays allow rapid and reliable measurement of the phosphorylation and the expression level of key signaling proteins in cell lysates. These assays are also suitable for tissue sample analysis to evaluate the in vivo efficacy of compounds on preclinical models. However, the proper preparation of lysates and working solutions using the right reagents is crucial to ensure you obtain the best results. Moreover, it is essential to include the appropriate controls to analyze and interpret the results correctly. This technical note provides procedures and guidelines to optimize your HTRF Cell Signaling assays on tissues, with a focus on sample preparation and data analysis. Please note that the procedure for tissue lysis and sample preparation is also suitable for western-blot experiments.