Phospho- and total AMPK assays for studying the metabolic master switch
Cisbio's cell-based homogeneous HTRF® kit for phospho-AMPK (Thr172) enables the quantitative detection of AMP-activated protein Kinase modulation, phosphorylated at Threonine 172. The HTRF® total AMPK assay kit is designed for the quantitative detection of total AMPK, phosphorylated and unphosphorylated, to normalize the phosphorylation status of AMPK with respect to its steady-state level in cells. The buffers of both HTRF® phospho- and total AMPK assays are compatible, enabling an analysis of the phosphorylated and the total protein populations from one lysate sample. With its central role in several metabolic pathways, AMPK is a significant target in diabetes research and an important tool for oncological and cardiovascular disease studies. The simple add-and-read protocol eliminates all wash steps for a faster analysis and easy miniaturization, while maintaining a high-quality specific output and offering enhanced convenience over ELISA or WB.