Homogeneous cell-based assay for phospho-ATF2 as a readout of JNK1 or p38MAPK
Cisbio's cell-based homogeneous HTRF® phospho-ATF2 assay (Thr71) enables both SAPK/JNK and p38 MAPK pathway readout, giving quantitative detection of the modulation of the transcription factor ATF2, phosphorylated on Threonine 71. ATF2 is also involved in PI3K/AKT, TNF and estrogen signaling, and amongst others ATF2 plays a role in insulin secretion, thyroid hormone release, substance dependence, viral carcinogenesis and infectious diseases. The kit is suitable for analysing pharmacological questions in cellular models. The simple add-and-read protocol, with no wash steps for a faster analysis, facilitates a wide variety of biological applications from basic reasearch to high throughput screening in oncology, diabetes, inflammation, cardiovascular, virology and CNS areas.