Phospho-c-Src (Tyr419) and Total c-Src assays to identify selective c-Src kinase inhibitors for cancer treatment
These cell-based assays are designed to monitor the expression level and the autophosphorylation of c-Src on Tyr419, which is a hallmark of its activation.
c-Src is a member of the Src kinase family and shares a high degree of sequence identity with the ten other members (Fyn, Lyn, Lck, Frk, Blk, Hck, Yrk, Fgr, Srm and Yes).
This protein is a membrane-associated, non-receptor tyrosine kinase. Its autophosphorylation on the residue Tyr419 signs its full activation.
c-Src kinase is derived from the proto-oncogene c-Src and regulates cell proliferation, differentiation, survival, morphology, adhesion and migration. It is over-expressed and highly activated in many human cancers (e.g. breast, colon and pancreatic), making it a promising target for cancer therapy.