Phospho- and total GSK3β and GSK3α detection as ubiquitous readout of major pathways
Cisbio's cell-based homogeneous HTRF® kits for phospho-GSK3 beta (Ser9), alpha (Ser21) and both total GSK3 assays enable separate detection of both isoforms of GSK3 and a multiple pathway readout. GSK3 is involved in PI3K/AKT signaling, Wnt/β-Catenin, Hedgehog, Notch, glycogen synthesis and cytoskeleton polymerization which control protein synthesis, cell proliferation, migration, inflammation, immune response, glucose regulation and apoptosis. The assays provide the quantitative detection of GSK3, the phosphorylated isoforms beta (Ser9) and alpha (Ser21), and the total proteins for normalization of the phospho-level detected. Phosphorylation of GSK3β Ser9 and GSK3α Ser21 normally decreases its enzymatic activity. The assays are suitable for cell-based screening for GSK3-upstream kinase inhibitors that result in increased GSK3 activity during cancer treatment, or GSK3 inhibitors for CNS disorders. The simple add-and-read protocol eliminates all wash steps for a faster analysis and easy miniaturization, while maintaining a high-quality specific output and offering enhanced convenience over ELISA or WB.