Sensitive phospho-H2AX detection, as a biomarker for DNA repair of double-strand breaks
Cisbio's cell-based homogeneous HTRF® phospho-H2AX Ser139 assay enables the quantitative detection of the histone variant H2AX, phosphorylated on serine 139. When double-stranded DNA breaks, either by ionizing radiation or endogenous physiological processes, H2AX becomes phosphorylated at Ser139. This modification, called gamma-H2AX (phospho-Ser139), serves as a sensitive biomarker for the detection of such breaks, localizing the site of DNA repair. Gamma-H2AX triggers cell cycle arrest and DNA damage response repair, which makes HTRF® phospho-H2AX Ser139 a pertinent assay to study genome stability, the cell cycle, DNA repair, and it serves as a biomarker for cancer, and to understand aging. The simple add-and-read protocol eliminates all wash steps for a faster analysis and permits easy miniaturization. The assay is amenable to low-volume formats for all throughputs and offers enhanced convenience over other immunoassay technologies.