HTRF® cell-based assays for studying the RAS-RAF-MEK cascade in the MAPK/ERK pathway
Cisbio's cell-based homogeneous phospho-MEK1 & phosho-MEK1/2 assays enable a MAPK signaling pathway readout, by allowing the quantitative detection of the MAP2K1 kinase phosphorylated on serine 218/222 or on serine 298. Phosphorylated by RAF, this dual specificity mitogen-activated protein kinase is responsible for the phosphorylation of ERK, one of the most important signaling nodes, and protein kinase in cell proliferation, survival and differentiation pathways.
The HTRF® total MEK1 assay kit is designed for the quantitative detection of total MEK1, phosphorylated and unphosphorylated, to normalize the phosphorylation status of MEK1.
The buffers of both HTRF® phospho- and total MEK1 assays are compatible, enabling an analysis of the phosphorylated and the total protein populations from one lysate sample.