Homogeneous cell-based assay for PDGF receptor β involved in angiogenesis and cancer
Cisbio's cell-based homogeneous HTRF® phospho-PDGFRβ assay (Tyr751) enables rapid, quantitative detection of the modulation of the platelet-derived growth factor receptor beta, phosphorylated on Tyrosine 751. PDGFRbeta signals and crosstalks via both the PI3K/AKT and the Ras/Raf MAPK pathways. This cell surface tyrosine kinase receptor ensures the binding of particular growth factors, which trigger the MAPK pathway leading to cell proliferation, cell differentiation and angiogenesis. When activated, PDGFRbeta forms a dimer and becomes phosphorylated, which makes the phospho-PDGFRβ assay a valuable tool in oncology research. This simple add-and-read assay is ideal for screening small molecule compounds or biologics directly in cells. The phospho-PDGFRβ ready-to-use kit contains all the reagents you need and offers enhanced convenience over other immunoassay technologies.