Total IRS2 assay to study pancreatic β-cell growth/survival and central nutrient sensing
Insulin Receptor Substrates 1 and 2 (IRS1/2) are the primary substrates of Insulin/IGF1 receptors, and mediate the activation of the downstream signaling pathways AKT and ERK. These lead to cell growth/survival and metabolic homeostasis.
While IRS1 is most important in adipose tissue and skeletal muscles, IRS2 is the predominant form in pancreatic β-cells and in the central nervous system (CNS).
Decreased IRS2 protein levels are associated with metabolic disorders (such as diabetes) and neurodegeneration. Conversely, IRS2 overexpression drives tumor motility and invasion.
This cell-based assay is designed to monitor the modulation of IRS2 protein levels.