The Total STING cellular assay monitors total STING, and can be used as a normalization assay with our phospho-STING kit. This kit is compatible with the buffers from the phospho-STING kit, so the same lysate can be used for analyses of both the phosphorylated and the total protein populations.
Following pathogen infection and binding of dsDNA to the cytoplasmic sensor cGAS, STING protein is phosphorylated by TBK1, enabling its binding to IRF3 which induces IFNs type 1 production. The STING pathway is then switched off by STING degradation involving autophagy.
In immuno-oncology, activating the STING pathway has shown promising anti-tumor effects in pre-clinical models and thus represents a therapeutic strategy to treat human cancer.