α-Tubulin and α-Tubulin Acetyl-K40 Assays Kits

Sandwich immunoassay kits designed for the rapid detection of alpha-Tubulin or alpha-Tubulin Acetyl-K40

Based on our TR-FRET homogeneous robust HTRF® technology, the alpha-Tubulin and alpha-Tubulin Acetyl-K40 sandwich immunoassay kits are designed for the rapid detection of total alpha-Tubulin and acetylation on K40 of alpha-Tubulin in cell lysates. This simple and robust procedure is suitable for miniaturization, and enables work with low sample sizes. The add & read protocol eliminates all wash steps for faster analysis. By detecting total and any modification of alpha-Tubulin, like acetylation on K40, this HTRF® kit gives a key readout of HDAC6 and other enzymes acting on alpha-Tubulin.

Assay principle

HTRF® - the homogeneous cell-based sandwich immunoassay

Alpha-Tubulin and alpha-Tubulin Acetyl-K40 are measured using sandwich immunoassays involving two specific anti-alpha Tubulin antibodies, respectively labelled with Europium cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of the alpha-Tubulin present in the sample.

Assay protocol

The protocol for alpha-Tubulin and alpha-Tubulin Acetyl-K40 assay is described here. Cells are plated, stimulated and next lysed in the same 96-well culture plate. Lysates are then transferred to the assay plate for the detection of Alpha-Tubulin by HTRF® reagents. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.

Part#, inserts & MSDS

Ordering Info

DescriptionCat. noProduct insertMSDS
Alpha-Tubulin acetyl-K40 kit - 500 tests 63ADK072PEG
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Alpha-Tubulin acetyl-K40 kit - 10,000 tests63ADK072PEH
Alpha-Tubulin kit - 500 tests 63ADK073PEG
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Alpha-Tubulin kit - 10,000 tests63ADK073PEH

Companion products

DescriptionCat. noProduct insertMSDS
Alpha-Tubulin acetyl-K40 kit - control lysate63ADK072TDA
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Alpha-Tubulin kit - control lysate63ADK073TDA
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Phospho-total protein lysis buffer #1 - 130 mL64KL1FDF

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